The Polycomb Protein Ezh2 Impacts on Induced Pluripotent Stem Cell Generation
作者： Xiaolei Ding , Xiaoying Wang , Stephanie Sontag , Jie Qin , Paul Wanek
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摘要: Reprogramming of somatic cells toward pluripotency involves extensive chromatin reorganization and changes in gene expression. Polycomb group (PcG) proteins are key regulators structure, cell identity, development. In this study, we investigated the impact Ezh2, a core subunit repressive complex 2 (PRC2), on generation induced pluripotent stem (iPS) cells. We found that Ezh2 expression is during iPS contain high levels mRNA protein. Importantly, shRNA knockdown reprogramming severely impairs generation. Mechanistically, acts at least part through repressing Ink4a/Arf locus, which represents major roadblock for Interestingly, established leaves self-renewal embryonic unaffected. Altogether, our results demonstrate critical efficient cell...
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