Manufacture of T cells using the Sleeping Beauty system to enforce expression of a CD19-specific chimeric antigen receptor

摘要: T cells can be reprogrammed to redirect specificity tumor-associated antigens (TAAs) through the enforced expression of chimeric antigen receptors (CARs). The prototypical CAR is a single-chain molecule that docks with TAA expressed on cell surface and, in contrast T-cell receptor complex, recognizes target independent human leukocyte antigen. bioprocessing generate CAR(+) has been reduced clinical practice based two common steps are accomplished compliance current good manufacturing practice. These (1) gene transfer stably integrate using viral and nonviral approaches (2) activating for proliferation by crosslinking CD3 or antigen-driven numeric expansion propagating (AaPCs). Here, we outline our approach Sleeping Beauty system selective propagation CD19-specific AaPCs.