Does seminal plasma PSP-I/PSP-II spermadhesin modulate the ability of boar spermatozoa to penetrate homologous oocytes in vitro?

摘要: Low concentration (0.15 mg per million of spermatozoa) seminal plasma-derived PSP-I/PSP-II spermadhesin heterodimer is able to preserve the viability highly extended boar spermatozoa. Whether spermatozoa also keep their fertilizing capacity not yet known. The present study evaluated effect exposing freshly and frozen-thawed (10 million/mL) (1.5 mg/mL) for 30 or 120 minutes on sperm character- istics outcome in vitro penetration immature (IM) matured (IVM) homologous oocytes, aiming identify this sper- madhesin as a suitable modulator sperm-handling protocols. Al- though exposure improved mo- tility without increasing levels acrosome exocytosis both spermatozoa, pretreat- ment did affect rates numbers oocyte when pretreated fresh were coincubated with IM IVM oocytes compared controls. When cryopreserved matozoa tested, however, already 30-minute preincubation showed significant blocking rate (from 90% 32%, P , .05) mean (2.9 1.6, .05). To disclose nature paradox, cleansed (by centrifugation saline bovine serum albumin through Percoll den- sity gradient separation) procedure repeated. Oocyte pene- tration (but number oocyte) increased (P either washed unwashed controls (53% vs 13% 31%, respec- tively). In addition, percentages polyspermic remained lower than control (38.5% 68.7%, respectively; con- clusion, results confirm that low dose sperma- dhesin preserves motility vitro. Although there was no obvious influence capability fresh- ly penetrate (ei- ther IVM), exerted deleterious fro- zen-thawed used oocytes. Such an cryopreservation seems certain extent reversible, since cleansing surface decreased, at least partially, effect, monospermic rates.