Characterization and prevalence of a novel white spot syndrome viral genotype in naturally infected wild crayfish, Procambarus clarkii, in Shanghai, China.
作者： Luzhi Jiang , Jinzhou Xiao , Liyuan Liu , Yingjie Pan , Shuling Yan
DOI: 10.1007/S13337-017-0394-4
关键词:
摘要: White spot syndrome virus (WSSV) infection is commonly detected by vp28-qPCR assay in wild crayfish, Procambarus clarkii, a widespread crustacean species the aquatic environment China. The virions of crayfish WSSV have been isolated and purified. Based on TEM observation, they exhibited morphological structures that are identical to known WSSV. In addition, major envelope protein VP28 was observed based Western blot analysis total structural proteins PCR amplification sequencing analyses variable regions ORF14/15, ORF23/24 ORF94, along with viral genomic phylogenomic analysis, indicated WSSV, named WSSV-CN-Pc, represents new genotype. Intramuscular injection bioassay revealed WSSV-CN-Pc as virulent Taiwan strain. characteristics dominant genotype, high load (107-108 WSSV/mg) prevalence (91.7%, 110 120 samples) surveyed crayfish. also detected, similar pattern farmed Litopenaeus vannamei shrimp shared ecological niches sampled Our results there horizontal transmission between nature. findings implicated farming should be integrated managed cautions order reduce risk spread circulation environment.
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