Vanadate stimulation of adenylyl cyclase: An index of tyrosine kinase vascular effects*

摘要: Background Beyond their mitogenic effects, hormones such as insulin, which activate receptor tyrosine kinases, regulate vascular tone. Further, we have demonstrated that kinase activation enhances adenylyl cyclase activation, a prominent mechanism mediates vasodilation. However, whether kinase–mediated human responses parallel cellular effects on activity is unknown. Methods and Results To assess responses, sensitivity to insulin was assessed with the dorsal hand vein linear variable differential transformer technique. Insulin infusion resulted in dose-dependent relaxation all subjects. Cellular were by means of insulinomimetic vanadate-mediated sensitization activity. Vanadate stimulated kinase–dependent enhancement function rat aortic smooth muscle cells, lymphocytes, endothelial cells. maximal insulin-mediated vasodilation significantly positively correlated lymphocyte activity. Conclusion Insulin-mediated Vanadate-mediated lymphocytes may represent an index effects. Clinical Pharmacology & Therapeutics (1999) 66, 275–281; doi: