Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA.

摘要: The transcriptional regulation of the sulfur amino acid pathway in Saccharomyces cerevisiae depends on a single activator, Met4p, whose function requires different combinations auxiliary factors Cbf1p, Met28p, Met31p and Met32p. first description how these cooperate to activate transcription was provided by identification Cbf1-Met4-Met28 complex which is assembled regulatory region MET16 gene. In this paper, we demonstrate that other pathways are used recruit Met4p 5' upstream two genes, MET3 MET28. cases, tethered DNA through alternative complexes associating with Met28p either or These formed over AAACTGTG sequence, cis-acting element found several MET genes. domain within mediates its interaction Met32p allowed vivo analysis specificity Met4p-containing complexes. results therefore co-regulation gene network may be gained molecular mechanisms. addition system exacerbates structural variety nucleoprotein bZIP factor can engaged.