Comparative genomic hybridization
作者： Donna G. Albertson , Daniel Pinkel
DOI: 10.1017/CBO9781139046947.004
关键词:
摘要: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers one or more genomes wherein repetitive sequences that bind multiple loci a reference chromosome spread either substantially removed and/or their signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for determining relative number copies subject portions thereof (for example, tumor cell) as function location those genome normal human genome). intensity(ies) from each labeled differences ratios between different compared determine position along spread. Amplifications, duplications deletions genome(s) can be detected. Also provided is method absolute all RNA DNA cell(s) cell population(s).
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