DNA BARCODING: CO1 DNA barcoding amphibians: take the chance, meet the challenge.

摘要: Although a mitochondrial DNA barcode has been shown to be of great utility for species identification and discovery in an increasing number diverse taxa, caution urged with its application one the most taxonomically vertebrate groups - amphibians. Here, we test three perceived shortcomings CO1 barcode's group Holarctic amphibians: primer fit, sequence variability overlapping intra- interspecific variability. We found that although priming regions were variable, able reliably amplify fragment from degenerate primers G-C residues at 3' end. Any overlap between variation our taxonomic sampling was due introgressive hybridization (Bufo/Anaxyrus), complex genetics (Ambystoma) or incomplete taxonomy (Triturus). Rates are not expected greater amphibians than other groups, thus problems using single gene will specific Therefore, conclude there is potential use reported date. A large-scale effort world, same primary region CO1, yield important findings science conservation.