Synthetic and biological approaches to map substrate specificities of proteases.

摘要: Proteases are regulators of diverse biological pathways including protein catabolism, antigen processing and inflammation, as well various disease conditions, such malignant metastasis, viral infection parasite invasion. The identification substrates a given protease is essential to understand its function this information can also aid in the design specific inhibitors active site probes. However, diversity putative peptide makes connecting downstream technically difficult time-consuming. To address challenge research, range methods have been developed identify natural map overall substrate specificity patterns proteases. In review, we highlight recent examples both synthetic that being used define so new protease-specific tools therapeutic agents be developed.