Detection of Metallothionein in Javanese Medaka (Oryzias javanicus), Using a scFv-Immobilized Protein Chip.
作者： Euiyeon Lee , Hyunjin Jeon , Chungwon Kang , Seonock Woo , Seungshic Yum
DOI: 10.3390/S18041069
关键词:
摘要: Environmental pollution by various industrial chemicals and biological agents poses serious risks to human health. Especially, marine contamination potentially toxic elements (PTEs) has become a global concern in recent years. Many efforts have been undertaken monitor the PTE of aquatic environment. However, there are few approaches available assess exposure organisms. In this research, we developed strategy evaluate heavy metal organisms, measuring expression levels metallothionein protein derived from Oryzias javanicus (OjaMT). OjaMT is biomarker because level increases upon exposure. The assay based on real-time, label-free surface plasmon resonance (SPR) measurement. Anti-OjaMT antibody anti-OjaMT single-chain fragment variable region (scFv) were used as detection probes. Two types SPR sensor chips fabricated, immobilizing or Cys3-tagged scFv (scFv-Cys3) controlled orientation tested for situ detection. Compared antibody-presenting chips, scFv-presenting showed improved performance, displaying enhanced sensitivity enabling semi-quantitative portable system combined with scFv-immobilized expected provide an excellent point-of-care testing that can target biomarkers real time.
core.ac.uk 
sci-hub.se 参考文章(25)
Ana Cristina Grodzki, Elsa Berenstein, Antibody purification: affinity chromatography - protein A and protein G Sepharose. Methods of Molecular Biology. ,vol. 588, pp. 33- 41 ,(2010) , 10.1007/978-1-59745-324-0_5
Ismail Ahmad, Daryoush Khodadoust, Metallothionein-Like Protein Levels in Java Medaka Fish (Oryzias javanicus) Exposed to Different Concentrations of Cadmium Walailak Journal of Science and Technology (WJST). ,vol. 11, pp. 883- 893 ,(2013) , 10.2004/WJST.V11I9.506
R W Olafson, W D McCubbin, C M Kay, Primary- and secondary-structural analysis of a unique prokaryotic metallothionein from a Synechococcus sp. cyanobacterium. Biochemical Journal. ,vol. 251, pp. 691- 699 ,(1988) , 10.1042/BJ2510691
Anna Farkas, Claudio Erratico, Luigi Viganò, Assessment of the environmental significance of heavy metal pollution in surficial sediments of the River Po Chemosphere. ,vol. 68, pp. 761- 768 ,(2007) , 10.1016/J.CHEMOSPHERE.2006.12.099
Ralf Lichtinghagen, Petra B Musholt, Michael Lein, Andreas Römer, Birgit Rudolph, Glen Kristiansen, Steffen Hauptmann, Dietmar Schnorr, Stefan A Loening, Klaus Jung, Different mRNA and Protein Expression of Matrix Metalloproteinases 2 and 9 and Tissue Inhibitor of Metalloproteinases 1 in Benign and Malignant Prostate Tissue European Urology. ,vol. 42, pp. 398- 406 ,(2002) , 10.1016/S0302-2838(02)00324-X
Humood A. Naser, Assessment and management of heavy metal pollution in the marine environment of the Arabian Gulf: A review Marine Pollution Bulletin. ,vol. 72, pp. 6- 13 ,(2013) , 10.1016/J.MARPOLBUL.2013.04.030
S.N. Sin, H. Chua, W. Lo, L.M. Ng, Assessment of heavy metal cations in sediments of Shing Mun River, Hong Kong Environment International. ,vol. 26, pp. 297- 301 ,(2001) , 10.1016/S0160-4120(01)00003-4
Kyoungmi Min, Deokho Jung, Yongkwon Jeon, Eunhee Jeoung, Youngeun Kwon, Site-specific and effective immobilization of proteins by Npu DnaE split-intein mediated protein trans-splicing reaction Biochip Journal. ,vol. 7, pp. 288- 294 ,(2013) , 10.1007/S13206-013-7312-7
Fahriye Ceyda Dudak, İsmail Hakkı Boyacı, Rapid and label-free bacteria detection by surface plasmon resonance (SPR) biosensors. Biotechnology Journal. ,vol. 4, pp. 1003- 1011 ,(2009) , 10.1002/BIOT.200800316
Seonock Woo, Seungshic Yum, Jee Hyun Jung, Won Joon Shim, Chang-Hoon Lee, Taek-Kyun Lee, Heavy metal-induced differential gene expression of metallothionein in Javanese medaka, Oryzias javanicus. Marine Biotechnology. ,vol. 8, pp. 654- 662 ,(2006) , 10.1007/S10126-006-6046-0