Microsatellites characteristics analysis and SSR marker development for Paphiopedilum hirsutissimum based on transcriptome sequencing
作者： Lin Chen , Hui Dong , Jianwen Wang , Longna Li , Meng Xu
DOI: 10.1017/S1479262117000375
关键词:
摘要: Paphiopedilum hirsutissimum (Lindl. ex Hook. f.) Stein (Orchidaceae) is a valuable but endangered ornamental plant. In the present study, microsatellites characteristics were analysed and 14 polymorphic simple sequence repeat (SSR) markers developed based on de novo transcriptome sequencing for P. using Illumina pair-end technology. A total of 7406 SSRs detected in 56,688 unigenes, with occurrence frequency 13.06% an average density one SSR per 5.42 kb; 150 primer pairs randomly selected from 2090 except mononucleotide repeats synthesized verification. These loci validated 32 individuals concentrated distribution centre, successfully amplified sets showing high level polymorphism. will be useful future studies genetic diversity, population structure make effective conservation strategy.
sci-hub.se 参考文章(10)
Dong-Mei Li, Wei Wu, Di Zhang, Xiao-Rong Liu, Xiao-Fei Liu, Yi-Jian Lin, None, Floral Transcriptome Analyses of Four Paphiopedilum Orchids with Distinct Flowering Behaviors and Development of Simple Sequence Repeat Markers Plant Molecular Biology Reporter. ,vol. 33, pp. 1928- 1952 ,(2015) , 10.1007/S11105-015-0886-6
Songjun Zeng, Kunlin Wu, Jaime A. Teixeira da Silva, Jianxia Zhang, Zhilin Chen, Nianhe Xia, Jun Duan, Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid Scientia Horticulturae. ,vol. 138, pp. 198- 209 ,(2012) , 10.1016/J.SCIENTA.2012.02.026
Songjun Zeng, Jia Wang, Kunlin Wu, Jaime A. Teixeira da Silva, Jianxia Zhang, Jun Duan, In vitro propagation of Paphiopedilum hangianum Perner Gruss Scientia Horticulturae. ,vol. 151, pp. 147- 156 ,(2013) , 10.1016/J.SCIENTA.2012.10.032
Chyuam-Yih Ng, Norihan Mohd. Saleh, In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies Plant Cell, Tissue and Organ Culture (PCTOC). ,vol. 105, pp. 193- 202 ,(2011) , 10.1007/S11240-010-9851-0
Siva P Kumpatla, Snehasis Mukhopadhyay, Mining and survey of simple sequence repeats in expressed sequence tags of dicotyledonous species. Genome. ,vol. 48, pp. 985- 998 ,(2005) , 10.1139/G05-060
Zhangying Wang, Jun Li, Zhongxia Luo, Lifei Huang, Xinliang Chen, Boping Fang, Yujun Li, Jingyi Chen, Xiongjian Zhang, Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas) BMC Plant Biology. ,vol. 11, pp. 139- 139 ,(2011) , 10.1186/1471-2229-11-139
Songjun Zeng, Weichang Huang, Kunlin Wu, Jianxia Zhang, Jaime A. Teixeira da Silva, Jun Duan, In vitro propagation of Paphiopedilum orchids Critical Reviews in Biotechnology. ,vol. 36, pp. 521- 534 ,(2015) , 10.3109/07388551.2014.993585
Zeng SongJun, Duan Jun, Tian RuiXue, Wu KunLing, Chen ZhiLin, Research Progress on Cross Breeding of Paphiopedilum Journal of Tropical and Subtropical Botany. ,vol. 18, pp. 459- 468 ,(2010)
D.M. Li, C.Y. Zhao, X.R. Liu, X.F. Liu, Y.J. Lin, J.W. Liu, H.M. Chen, F.B. Lǚ, De novo assembly and characterization of the root transcriptome and development of simple sequence repeat markers in Paphiopedilum concolor. Genetics and Molecular Research. ,vol. 14, pp. 6189- 6201 ,(2015) , 10.4238/2015.JUNE.9.5
Meng Xu, Xin Liu, Jian-Wen Wang, Shi-Yuan Teng, Ji-Qing Shi, Yuan-Yuan Li, Min-Ren Huang, Transcriptome sequencing and development of novel genic SSR markers for Dendrobium officinale Molecular Breeding. ,vol. 37, pp. 18- ,(2017) , 10.1007/S11032-016-0613-5