Targeted use of fluorescence in situ hybridization (FISH) in cytospin preparations: results of 298 fine needle aspirates of B-cell non-Hodgkin lymphoma.

摘要: BACKGROUND: Fluorescence in situ hybridization (FISH) results from fine needle aspirates (FNA) of B-cell non-Hodgkin lymphomas (NHLs) were reviewed to 1) investigate the value added by using specific gene rearrangement probes lymphoma diagnosis, prognosis, and subtyping; 2) evaluate prevalence cytogenetic alterations other than translocations. METHODS: FISH assays performed on cytospin preparations NHL FNAs over a 6-year period (2003-2009) selected. Immunophenotyping, clinical data, cytomorphologic data according current World Health Organization (WHO) classification system. Hybridized probes, purpose for assay (subtyping or prognosis), abnormalities observed retrieved cytology reports. Data was categorized rearrangements chromosomal abnormalities. RESULTS: Successful obtained 284 (95.3%) 298 cases 282 patients. Abnormalities found 216 (76%) 68 (24%) did not show alteration. Among submitted subtyping, 198 showed FISH-positive results, 122 (61.6%) as follows: follicular 82, mantle cell 21, marginal zone 3, “dual hit” 13, Burkitt 3. In 21 cases, useful prognosis. Nonspecific alone combination with translocations 98 cases. CONCLUSIONS: FISH confirmation subclasses may also provide valuable prognostic information. Cytogenetic frequently could supportive evidence definitive diagnosis FNA. Cancer (Cancer Cytopathol) 2010. © 2010 American Society.