Applying conserved peptides of NS1 Protein of avian influenza virus to differentiate infected from vaccinated chickens

摘要: Avi an influenza (AI) is a highly contagious disease in poultry and outbreaks can have dramatic economic health implications. For effective surveillance, rapid sensitive assays are needed to detect antibodies against AI virus (AIV) proteins. In order support eradication efforts of avian infections poultry, the implementation “DIVA” vaccination strategies, enabling Differentiation infected from Vaccinated Animals been recommended by international organizations. A system, based on detection Non-Structural (NS1) protein has proposed enable field exposure vaccinated flocks, through this detection, flocks may be properly managed. project we used two conserved peptides NS1 develop peptide ELISA method. This could screen sera due their titer antibody. Following experimentally infection vaccinate chickens, were detected enzyme-linked immuno sorbent assay (ELISA). These findings indicate that there significant difference viral replication resulting variation production NS1, as peptide- used. results demonstrate specific for anti diagnostic value industries.