Controlled auxin biosynthesis and transport are important for developmental decisions in the early diverging land plant Physcomitrella patens

摘要: The aim of this thesis was to increase our understanding the importance plant hormone auxin in land evolution. role a number regulatory network components developmental decisions during haploid phase life cycle model moss Physcomitrella patens, which belongs early diverging bryophyte group plants, investigated. We show that SHI/STY transcriptional activators regulation biosynthesis rates seed plants is conserved moss. amount and synthesis are reduced PpSHI knockout mutants, defects these mutants can be mimicked by reducing active expression domain, several homologues YUC TAA1/TAR genes, encode main enzymes were activated when PpSHI2 transiently expressed. Our data reveal genes induce biosynthesis, their domains largely overlap with two PpSHIs certain stages cycle. also directly or indirectly activates involved polar transport (PAT) PpPINA, PpPINB PpLAXB. efflux function PIN proteins have been evolution they, together regulators, important for determining developmentally regulated decisions, such as switch between cell types filamentous stage In addition, PIN-mediated distribution synthesised leaves plays an wave expansion. local crucial steps reproductive organ development, egg maturation, clearance canal cavity, well apical opening both male female organs allowing sperm release entrance into cavity. If remains studied. This demonstrates operational cell-fate already has played key complex structures.