Anti-oxidant activity of spermine and spermidine re-evaluated with oxidizing systems involving iron and copper ions

摘要: Abstract This study was designed to investigate the direction of redox reactions spermine and spermidine in presence iron copper. The activity assessed using a variety methods, including their ability to: (1) reduce Fe3+ Fe2+ ions; (2) protect deoxyribose from oxidation by Fe2+–ethylene diaminetetraacetic acid, Fe3+–ethylene acid systems with without H2O2; (3) DNA damage caused Cu2+–H2O2, Fe2+–H2O2 ascorbic acid; (4) inhibit H2O2-peroxidase-induced luminol dependent chemiluminescence; (5) scavenge diphenyl-picryl-hydrazyl radical. Spermine at concentration 1 mM reduced 1.8 ± 0.3 2.5 ± 0.1 nmol ions during 20 min incubation. Both polyamines enhanced oxidation. highest enhancement 7.6-fold degradation found for combination acid. An 10 mM decreased CuSO4–H2O2–ascorbic acid- FeSO4–H2O2–ascorbic-induced 73 ± 6, 69 ± 4% 90 ± 5, 53 ± 4%, respectively. They did not CuSO4–H2O2 FeSO4–H2O2. apparently increased CuSO4–H2O2-dependent injury DNA. Polyamines attenuated chemiluminescence. Total light emission specimens containing or 85.3 ± 1.5 87 ± 3.6%. During incubation decomposed 8.1 ± 1.4 9.2 ± 1.8% These results demonstrate that well known anti-oxidant properties may act as pro-oxidants enhance oxidative components free H2O2.