Azidopropylvinylsulfonamide as a New Bifunctional Click Reagent for Bioorthogonal Conjugations: Application for DNA-Protein Cross-Linking.
作者： Jitka Dadová , Milan Vrábel , Matej Adámik , Marie Brázdová , Radek Pohl
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摘要: N-(3-Azidopropyl)vinylsulfonamide was developed as a new bifunctional bioconjugation reagent suitable for the cross-linking of biomolecules through copper(I)-catalyzed azide-alkyne cycloaddition and thiol Michael addition reactions under biorthogonal conditions. The is easily clicked to an acetylene-containing DNA or protein then reacts with cysteine-containing peptides proteins form covalent cross-links. Several examples bioconjugations ethynyl- octadiynyl-modified peptides, p53 protein, alkyne-modified human carbonic anhydrase are given.
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