Centrifugal force induces human ligamentum flavum fibroblasts inflammation through activation of JNK and p38 pathways

摘要: Inflammation has been proposed to be an important causative factor in ligamentum flavum hypertrophy. However, the mechanisms of mechanical load on inflammation remain unclear. In this study, we used vitro model human fibroblasts subjected centrifugal force elucidate effects cultured fibroblasts; further studied its molecular and biochemical mechanisms. Human were obtained from six patients undergoing lumbar spine surgery. Monolayer cultures different magnitudes forces. Cell viability, cell death, response, response forces analyzed. It was found that stress significantly suppressed viability without inducing death. Centrifugal at 67.1 g/cm(2) for 60 min increases production prostaglandin E2 nitric oxide as well gene expression proinflammatory cytokines, including interleukin (IL)-1α, IL-1β IL-6, showed force-dependent induction cyclooxygense-2 inducible NO synthase required JNK p38 mitogen-activated protein kinase, but not ERK 1/2 activities. This study suggested does induce inflammatory responses fibroblasts. The activation both kinase mechanotransduction cascades is a crucial intracellular mechanism mediates cyclooxygense-2/prostaglandin synthase/nitric production.