Epidermal Growth-Factor – Induced Transcript Isoform Variation Drives Mammary Cell Migration

摘要: Signal-induced transcript isoform variation (TIV) includes alternative promoter usage as well splicing and polyadenylation of mRNA. To assess the phenotypic relevance signal-induced TIV, we employed exon arrays breast epithelial cells, which migrate in response to epidermal growth factor (EGF). We show that EGF rapidly – within one hour induces widespread TIV a significant fraction transcriptome. Importantly, characterizes many genes display no differential expression upon stimulus. In addition, similar EGF-dependent changes are shared by panel mammary cell lines. A functional screen, utilized isoform-specific siRNA oligonucleotides, indicated several isoforms play essential, non-redundant roles EGF-induced migration. Taken together, our findings highlight importance rapid evolvement extracellular signals.