Apparatus for culture of biological cells and tissues

摘要: 863,707. Apparatus for cultivating biological cells C April 23, 1958], Nos. 26639/57 and 12863/58. Class 81 (1). the culture of tissues, e.g. a strain mouse fibroplasts, comprises two Circuits " which may be interconnected by means connecting-path 13 in Figure opened or closed. The first circuit 10 reservoir 11 liquid nutrient medium second 12 contains growth vessel 14 tissues are maintained suspension at suitable temperature thermostatically controlled bath. material both circuits circulated peristaltic pump 15. Included apparatus whereby batches automatically pumped from wherein it is simultaneously replaced fresh predetermined intervals such amounts that concentration cultivated remains substantially constant. This done, example, solenoid-operated tap 28 activated an electrical uniselector relay operates after given number impulses switch operated cam on shaft subsequently re-set until impulse causes to operate again. removal replacement alternatively made more directly dependent amount present utilizing photo-electric cell association with produces required when turbidity reaches value. Alternatively, pH using as above 37 receiving radiation light source 36 through filter 38A glass 31 forms part closed supplementary 33 containing physiological salt solution indicator, phenol red, linked primary (growth) dialysis 32 fluid secondary separated semi-permeable membrane. In this case chosen transmit limited wave-length effected indicator changes colour. form shown Fig. 5, limbs 17 18 connect circuit, gas, air 5% CO 2 , passed space 22 23.