Signal transduction of c-Kit receptor tyrosine kinase in CHRF myeloid leukemia cells.
作者： Sebastian Scholl , Cornelia Kirsch , Frank D. B�hmer , Reinhard Klinger
DOI: 10.1007/S00432-004-0602-7
关键词:
摘要: The tyrosine kinase receptor c-Kit (stem cell factor receptor, CD117) is a potential target for signal transduction therapy in different cancers. In this study we investigated CHRF cells, megakaryoblastic line of Acute Myeloid Leukemia (FAB M7). We characterized the interactions between and PI 3-kinase (p85) after stimulation with SCF factor) as well regulation SHP-1 SHP-2 associated Kit line. Stimulation leads to significant increase interaction p85 activity. Interestingly, using kinds substances (AG 1295, CGP 53716) inhibit activity blocked activation c-Kit, but association still increased even when was completely blocked. contrast, other known partners SHP-2, exhibited basal no change could be detected cells or treatment inhibitors. Therefore, suggest that p85, SHP-1, can, at least part, occur kinase-activity independent manner. necessary receptor-associated 3-kinase.
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