Importance of intermediary transitions and waveform in the enzyme–electric field interaction

作者: Ernesto Federico Treo , Carmelo José Felice

DOI: 10.1016/J.BIOELECHEM.2008.01.001

关键词:

摘要: The current theory of enzymes and electric field interaction does not account for all the observed data since we could observe non-linear behavior cell suspensions as anticipated by other authors. In our case, used a pure sinusoidal source, however experiments that do responses sum central its harmonics frequencies. As result, suggest enzyme are favored when has non-strictly waveform, this is related to true intermediate transitions during catalytic cycle. Therefore, developed an iterative model process based on previous models actual trends. well verified simulations showed that, non-symmetrical enzyme, energy can harvest maximal non fields.

参考文章(39)
Gene A. Scarborough, Molecular mechanism of the P-type ATPases. Journal of Bioenergetics and Biomembranes. ,vol. 34, pp. 235- 250 ,(2002) , 10.1023/A:1020211016696
Gene A. Scarborough, Why we must move on from the E1E2 model for the reaction cycle of the P-type ATPases. Journal of Bioenergetics and Biomembranes. ,vol. 35, pp. 193- 201 ,(2003) , 10.1023/A:1024641413205
E H Serpersu, T Y Tsong, Activation of electrogenic Rb+ transport of (Na,K)-ATPase by an electric field. Journal of Biological Chemistry. ,vol. 259, pp. 7155- 7162 ,(1984) , 10.1016/S0021-9258(17)39851-4
C M Pickart, W P Jencks, Energetics of the calcium-transporting ATPase. Journal of Biological Chemistry. ,vol. 259, pp. 1629- 1643 ,(1984) , 10.1016/S0021-9258(17)43455-7
K. E. Van Holde, W. Curtis Johnson, Pui Shing Ho, Principles of physical biochemistry ,(1998)
B E Knox, T Y Tsong, Voltage-driven ATP synthesis by beef heart mitochondrial F0F1-ATPase. Journal of Biological Chemistry. ,vol. 259, pp. 4757- 4763 ,(1984) , 10.1016/S0021-9258(17)42911-5
Andrew M. Woodward, Douglas B. Kell, On the nonlinear dielectric properties of biological systems: Saccharomyces cerevisiae Journal of Electroanalytical Chemistry. ,vol. 299, pp. 83- 100 ,(1990) , 10.1016/0302-4598(90)85013-8
D S Liu, R D Astumian, T Y Tsong, Activation of Na+ and K+ pumping modes of (Na,K)-ATPase by an oscillating electric field. Journal of Biological Chemistry. ,vol. 265, pp. 7260- 7267 ,(1990) , 10.1016/S0021-9258(19)39108-2
H. V. Westerhoff, Y. Chen, Stochastic free energy transduction Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 3222- 3226 ,(1985) , 10.1073/PNAS.82.10.3222
H. V. Westerhoff, T. Y. Tsong, P. B. Chock, Y. D. Chen, R. D. Astumian, How enzymes can capture and transmit free energy from an oscillating electric field. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 4734- 4738 ,(1986) , 10.1073/PNAS.83.13.4734