Cyclic stretch enhances reorientation and differentiation of 3-D culture model of human airway smooth muscle.

摘要: Activation of airway smooth muscle (ASM) cells plays a central role in the pathophysiology asthma. Because ASM is an important therapeutic target asthma, it beneficial to develop bioengineered models available for assessing physiological and biophysical properties cells. In condition vivo, are surrounded by extracellular matrix (ECM) exposed mechanical stresses such as cyclic stretch. We utilized 3-D culture model human embedded type-I collagen gel. further examined effects stretch, which mimics tidal breathing, on cell orientation expression contractile proteins within exhibited tissue-like structure with actin stress fiber formation intracellular Ca2+ mobilization response methacholine. Uniaxial stretching enhanced alignment nuclei fibers Moreover, mRNAs α-smooth actin, calponin, myosin heavy chain 11, transgelin stretched was significantly higher than that under static condition. Our findings suggest force interaction ECM affects development construct differentiation phenotype model.