Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq

摘要: Chromatin immunoprecipitation (ChIP) is used to evaluate the interaction of proteins and genomic DNA. In eukaryotic cells, DNA highly compacted with evolutionarily conserved histone (which together form nucleosome) other chromosomal-associated chromatin structure. structure dynamically regulated by several mechanisms including transcription factor binding various posttranslational modifications proteins. The can be affected environmental factors, such as those that induce differentiation or promote self-renewal in stem cells. Using very specific antibodies, one amino acids within histones each these associated a distinct DNA-templated process, transcription. Therefore, determining location factors yield important insights into DNA-associated activities are occurring at particular region genome time. ChIP followed high-throughput sequencing (ChIP-Seq) provides means rapidly determine precise sites on genome-wide scale. Genome-wide mapping chromatin-associated have already begun reveal responsible for regulating pattern gene expression mouse embryonic However, similar studies human cells currently lacking due difficulty obtaining large number purified typically required ChIP-Seq experiments. Here, we describe detailed method locations using only million