Viability, prostaglandin E2 production, and protein handling in normal and inflamed human colonic mucosa cultured for up to 48 h in vitro.

摘要: Human colonic mucosa obtained from colon cancer resections ('normal') and colectomies owing to ulcerative colitis (inflamed) were cultured for up 48 h in vitro. The 3H-leucine incorporation normal tissue decreased 52% (p < 0.001) at compared with 24 h. protein synthesis but not inflamed explants was significantly 0.01) improved h, reaching 72% of the 24-h value, on additions insulin protease inhibitors aprotinin, soyabean trypsin inhibitor, Nα-tosyl-L-lysine chloromethyl ketone culture medium. Inflamed had significant losses 15% after 29% culture, excretion precipitable 3H-leucine-labelled proteins could be as high 20%/24 A slight loss observed labelled very low (3%). prostaglandin E2 (PGE2) production both displayed an increasing non-linear pattern time ...