Isolation of Porcine Circovirus-like Viruses from Pigs with a Wasting Disease in the USA and Europe:

摘要: Samples of lung, liver, kidney, pancreas, spleen, and lymph node from pigs with postweaning multisystemic wasting syndrome California (USA) samples mesenteric nodes similarly diseased Brittany (France) were examined by light microscopy, in situ hybridization (ISH), and/or virus isolation. Whole genomic probes for porcine circovirus (PCV) chicken anemia (CAV) used ISH. Tissue homogenate supernatants inoculated onto PK/15 cells isolation, the presence viral antigen particles was verified indirect immunofluorescence, ISH, electron microscopy. Histologic examination lung revealed interstitial pneumonia, alveolar epithelial hyperplasia, basophilic nuclear cytoplasmic inclusions mononuclear cell infiltrates various pulmonary cells. Granulomatous lymphadenitis syncytial typified lesions seen France. PCV-like nucleic acid detected ISH node, liver California. Positive signal also obtained sections Probes CAV consistently negative. cultures preparations France had positive fluores- cence staining PCV using pooled polyclonal pig sera hyperimmune rabbit serum variable a panel 7 monoclonal antibodies specific culture contaminant PCV. cultures. Cytopathic effect not observed. Electron microscopic 17-nm morphologically consistent No other Although analysis definitive identification these isolates remains to be done, evidence provided strongly suggests that tissue are closely related to, although antigenically distinct from, original contaminant. Porcine first as continuous kidney line (PK/ 15). 17 Since this initial identification, has been shown contain single-stranded circular DNA genome 1.76 kb 13,15 is now classified new family, Circoviridae. 11 Serum antibody demonstrated Germany, 15,16 Canada, New Zealand, 10 Great Britain, 8 Northern Ireland. 4 Experimental infections in- ocula, derived contaminated cultures, have failed produce clinical disease. 3,16 several recovered still- born piglets Ireland, 3 potential