Oxygenated form of protocatechuate 3,4-dioxygenase, a non-heme iron-containing dioxygenase, as reaction intermediate.

摘要: Abstract A short-lived new spectral species of protocatechuate 3,4-dioxygenase, a trivalent non-heme iron-containing enzyme, was observed in the early stage reaction. This characterized by broad absorption band with maximum between 500 and 520 mµ, distinct from those enzyme or enzyme-protocatechuic acid complex. It could be demonstrated only presence both protocatechuic molecular oxygen. The rate constant for decomposition this determined stopped flow analysis agreed quite well turnover number as over-all During steady state reaction, similar shown substrate analogues which were metabolized more slowly than acid. Based on these findings, interpreted to represent an oxygenated form namely ternary complex oxygen, substrate, obligatory intermediate