MOLECULAR COMPARATIVE ANALYSIS OF MYCOPLASMA GALLISEPTICUM FIELD AND VACCINE STRAINS IN EGYPT

作者: Yousreya M. Hashem , Sabry I. Eissa , Rabab A. Khalifa , Mohamed K. Refaie , Ashraf M. Metwally

DOI:

关键词:

摘要: Mycoplasma gallisepticum (MG) infection is one of the most important causes economic losses in commercial poultry Egypt spite different control strategies applied. The current study dealt with Egyptian MG isolates obtained from flocks ages and showing health status. Tracheal samples selected breeder were subjected to real time polymerase chain reaction (rt PCR). DNA extracts positive rt PCR sent for mgc2 gene sequencing. In addition, sequences published strains GenBank molecular characterization comparison reference field vaccine strains. sequence analysis showed that there are nucleotides subsequent amino acids changes gained by which have led significant antigenic alterations. These profile could affect control.

参考文章(19)
K.M. OSMAN, M.M. ALY, Z.M.S. AMIN, B.S. HASAN, Mycoplasma gallisepticum: an emerging challenge to the poultry industry in Egypt. Revue Scientifique Et Technique De L Office International Des Epizooties. ,vol. 28, pp. 1015- 1023 ,(2009) , 10.20506/RST.28.3.1940
K.G. WHITHEAR, Control of avian mycoplasmoses by vaccination. Revue Scientifique Et Technique De L Office International Des Epizooties. ,vol. 15, pp. 1527- 1553 ,(1996) , 10.20506/RST.15.4.985
S. H. Kleven, H.-H. Fan, K. S. Turner, Pen Trial Studies on the Use of Live Vaccines to Displace Virulent Mycoplasma gallisepticum in Chickens Avian Diseases. ,vol. 42, pp. 300- 306 ,(1998) , 10.2307/1592480
Saad Gharaibeh, Victoria Laibinis, Ruth Wooten, Lisa Stabler, Naola Ferguson-Noel, Molecular Characterization of Mycoplasma gallisepticum Isolates from Jordan Avian Diseases. ,vol. 55, pp. 212- 216 ,(2011) , 10.1637/9526-091510-REG.1
Shmuel Razin, David Yogev, Yehudith Naot, Molecular Biology and Pathogenicity of Mycoplasmas Microbiology and Molecular Biology Reviews. ,vol. 62, pp. 1094- 1156 ,(1998) , 10.1128/MMBR.62.4.1094-1156.1998
Desmond G. Higgins, Paul M. Sharp, Fast and sensitive multiple sequence alignments on a microcomputer Bioinformatics. ,vol. 5, pp. 151- 153 ,(1989) , 10.1093/BIOINFORMATICS/5.2.151
Leka Papazisi, Timothy S. Gorton, Gerald Kutish, Philip F. Markham, Glenn F. Browning, Di Kim Nguyen, Steven Swartzell, Anup Madan, Greg Mahairas, Steven J. Geary, The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low). Microbiology. ,vol. 149, pp. 2307- 2316 ,(2003) , 10.1099/MIC.0.26427-0
Florian Winner, Renate Rosengarten, Christine Citti, In Vitro Cell Invasion of Mycoplasma gallisepticum Infection and Immunity. ,vol. 68, pp. 4238- 4244 ,(2000) , 10.1128/IAI.68.7.4238-4244.2000