Introns boost transgene expression in Drosophila melanogaster

摘要: Using Drosophila as a host, we have examined the effect that presence of an intron has on accumulation processed transgene mRNA. To provide model system was free from major position effects, two fish antifreeze protein (AFP) transgenes were arranged in divergent transcriptional orientation central yolk promoter/enhancer region and introduced into flies by P-element transformation and/or mobilization. In this way organization both structural genes promoter elements mimicked their natural arrangements. When one member AFP pair had its single 180 bp deleted, there 2- to 11-fold (average 5-fold) decrease mRNA level compared generated control gene containing intervening sequence. deleted replaced 70 sequence originating 1 gene, restored original level. Even for streamlined genome Drosophila, where number size are generally reduced mammals, appears facilitate accumulation.