Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber
作者： Hongjian Wan , Zhenguo Zhao , Chuntao Qian , Yihu Sui , Ahmed Abbas Malik
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摘要: Quantitative real-time polymerase chain reaction (QRT-PCR) has become one of the most widely used methods for gene expression analysis. However, the expression profile of a target …
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Steven R. Kain, Methods and protocols. Methods of biochemical analysis. ,vol. 47, pp. 407- 421 ,(2005) , 10.1002/0471739499.OTH1
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Mukesh Jain, Aashima Nijhawan, Akhilesh K. Tyagi, Jitendra P. Khurana, Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR Biochemical and Biophysical Research Communications. ,vol. 345, pp. 646- 651 ,(2006) , 10.1016/J.BBRC.2006.04.140
Stephen Bustin, Sina Dorudi, Molecular assessment of tumour stage and disease recurrence using PCR-based assays Molecular Medicine Today. ,vol. 4, pp. 389- 396 ,(1998) , 10.1016/S1357-4310(98)01324-0
Tomasz Czechowski, Mark Stitt, Thomas Altmann, Michael K. Udvardi, Wolf-Rüdiger Scheible, Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis Plant Physiology. ,vol. 139, pp. 5- 17 ,(2005) , 10.1104/PP.105.063743
Bo-Ra Kim, Hee-Young Nam, Soo-Un Kim, Su-Il Kim, Yung-Jin Chang, Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice Biotechnology Letters. ,vol. 25, pp. 1869- 1872 ,(2003) , 10.1023/A:1026298032009
Jiayu Ding, Junwei Jia, Litao Yang, Haibo Wen, Chengmei Zhang, Wenxuan Liu, Dabing Zhang, Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. Journal of Agricultural and Food Chemistry. ,vol. 52, pp. 3372- 3377 ,(2004) , 10.1021/JF049915D
Michael W. Pfaffl, Ales Tichopad, Christian Prgomet, Tanja P. Neuvians, Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations. Biotechnology Letters. ,vol. 26, pp. 509- 515 ,(2004) , 10.1023/B:BILE.0000019559.84305.47
Claus Lindbjerg Andersen, Jens Ledet Jensen, Torben Falck Ørntoft, None, Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets Cancer Research. ,vol. 64, pp. 5245- 5250 ,(2004) , 10.1158/0008-5472.CAN-04-0496
David G Ginzinger, Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Experimental Hematology. ,vol. 30, pp. 503- 512 ,(2002) , 10.1016/S0301-472X(02)00806-8