Derivatization of Colloidal Gold Nanoparticles Toward Their Application in Life Sciences11This chapter is an adopted version based on the PhD thesis of Dominik Hühn as submitted at the Philipps Universität Marburg.

摘要: Abstract In this chapter, the derivatization of colloidal gold nanoparticles (Au NPs) in terms functionalization and characterization is described. This chapter hereby focuses particular on Au NPs which consist an inorganic core approximately 5 nm diameter, stabilized by hydrophobic surface molecules. Transfer to aqueous environments (an indispensable necessity for biomedical applications) has been achieved coating them with amphiphilic polymer, generates water solubility moreover gives ability further functionalization. The physicochemical properties such are verified within different purposes. First, several fundamental intrinsic quantified, including establishment pH titration as tool. It found that carboxylic groups, responsible stabilization, partly have (like their pKa) compared free-standing acids. These findings crucial stabilization nanoparticles, well Second, two species fluorescently labeled NPs, differ first order only net charge, employed study charge-dependent interaction biological systems, protein adsorption vitro internalization living cells. For these polymers variable charge developed. formation so-called corona around described, far-reaching impacts cell abilities. data demonstrate positively charged show higher toxicity. Third, highlight one potential application, modified toward sensing applications ion-sensitive dyes. Positively a Cl−-sensitive dye negatively Zn2+-sensitive dye. summary, will describe extension existing techniques nanoparticle derivatization, particularly regarding new types functional tuning particles, it outline comprehensive ranging from verification intrinsic, applications.