Screening of Nutritional Parameters for the Production of Protease from Aspergillus Oryzae

摘要: Production of protease enzyme by fungus Aspergillus oryzae was investigated. The proteolytic activity observed when the grown in medium containing glucose, malt extract, yeast peptone, K2HPO4, MgSO4 and FeSO4. present paper describes screening media components fermentation conditions shake flask. organism utilized carbon sources fructose, sucrose, lactose, dextrin starch among them glucose found to be best source, for nitrogen various inorganic organic were investigated peptone is source. 1% cottonseed followed 2% Soya bean meal inducer. With optimized two-fold increase production. growth depends on concentration carbon, salt solution, where as production also influenced culture time, pH interaction between these two variables.