Organizing neuronal ER-PM junctions is a conserved nonconducting function of Kv2 plasma membrane ion channels
作者： Michael Kirmiz , Stephanie Palacio , Parashar Thapa , Anna N. King , Jon T. Sack
DOI: 10.1101/296731
关键词:
摘要: Endoplasmic reticulum (ER) and plasma membrane (PM) form junctions crucial to ion lipid signaling homeostasis. The Kv2.1 channel is unique among PM proteins in organizing ER-PM junctions. Here, we show that this function conserved between Kv2 family members differ their biophysical properties, modulation cellular expression. Manipulation of actin cytoskeleton surrounding affects spatial organization. Kv2-containing overlap with those formed by canonical tethers. junction organization channels unchanged point mutations eliminate conduction, but abolished clustering without impacting function. Kv2.2 distinct lacking the reversible present Kv2.1. Brain neurons double knockout mice have altered junctions, demonstrating a vivo for from role as ion-conducting shaping neuronal excitability.
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