Analysis of baicalein, baicalin and wogonin in Scutellariae radix and its preparation by microemulsion electrokinetic chromatography with 1-butyl-3-methylimizolium tetrafluoborate ionic liquid as additive

作者: Huige Zhang , Kan Tian , Jianghong Tang , Shengda Qi , Hongli Chen

DOI: 10.1016/J.CHROMA.2006.08.016

关键词:

摘要: Microemulsion electrokinetic chromatography (MEEKC) using 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM-BF4) ionic liquid (EL) as additive was developed for the analysis of baicalin, wogonin and baicalein in Scutellariae radix its preparation. After conducting a series optimizations, baseline separation obtained analytes within 5 min under optimum conditions (sodium dodecyl sulfate (SDS) 0.88% (m/v) ethyl acetate 0.8% (v/v) butan-1-ol 0.2% buffer composition were 25% acetonitrile (v/v), 7.5 mm BMIM-BF4 10mM NaH2PO4, pH 8.2, applied voltage 17.5kV detection at 254nm), method has been successfully to determination quantification extracts S. (cooked), (raw) Qingfeiyihuowan which preparation including radix. (c) 2006 Elsevier B.V. All rights reserved.

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