Characterization and validation studies of powerPlex 2.1, a nine-locus short tandem repeat (STR) multiplex system and penta D monoplex.

摘要: In order to increase the power of discrimination for human identification purposes, a nine-locus short tandem repeat (STR) multiplex, GenePrint® PowerPlex™ 2.1 system (PowerPlex™ 2.1) developed by Promega Corporation and separate pentanucleotide-repeat locus, Penta D, were tested. This megaplex includes highly polymorphic loci FGA, TPOX, D8S1179, vWA, E, D18S51, D21S11, TH01, D3S1358 may be used in combination with eight-locus STR 1.1 1.1) that has been previously developed. Three loci, TH01 have included both systems quality control purposes. As 1.1, is also based on two-color detection fluorescent-labeled DNA products amplified polymerase chain reaction (PCR) provides valuable tool accurate rapid allele determination. The primer sequences 2.1/Penta D are presented this report. To meet “Quality Assurance Standards Forensic Testing Laboratories” (FBI), we tested efficiency reproducibility several validation studies conducted as joint project among seven laboratories. Validation tests concordance studies, sensitivity, species specificity determination, well performance forensic environmentally impacted samples. results produced from these demonstrated consistency reliability system.