Local changes in chromatin accessibility and transcriptional networks underlying the nitrate response in Arabidopsis roots

摘要: Abstract Transcriptional regulation, determined by the chromatin structure and regulatory elements interacting at promoter regions, is a key step in plant responses to environmental cues. Nitrate (NO3−) nutrient signal that regulates expression of hundreds genes Arabidopsis thaliana. Here, we integrate mRNA sequencing, genome-wide RNA polymerase II (RNPII), immunoprecipitation DNase sequencing datasets establish relationship between RNPII occupancy accessibility response NO3− treatments roots. Genomic footprinting allowed us identify in vivo controlling gene treatments. NO3−-modulated transcription factor (TF) footprints are important for rapid increase transcript accumulation over time. We mapped TF interactions functionally validated role NAP, an NAC-domain containing TF, as new transport. Taken together, our study provides comprehensive view transcriptional networks