Characterization and segmental distribution of 125I-Bolton-Hunter-labeled substance P binding sites in rat spinal cord.
作者： CG Charlton , CJ Helke
DOI: 10.1523/JNEUROSCI.05-05-01293.1985
关键词:
摘要: Substance P (SP) is widely distributed in the spinal cord and has been implicated as a neurotransmitter several neuronal systems. To investigate SP receptors cord, 125I-Bolton- Hunter-SP (125I-BH-SP) was used to identify characterize binding sites for peptide. The of 125I-BH-SP had following characteristics: high affinity; time, temperature, membrane concentration dependent; reversible; saturable. IC50 whole 0.46 nM compared with 0.95, 60, 150 physalaemin, eledoisin, kassinin. Four putative antagonists were less than 0.0001 times potent inhibiting binding. IC50s 5, 7.5, 7.0, 45 microM D-Pro2, D-Trp7,9-SP; D-Phe7, D-Trp9-SP; D-Arg1, D- Trp7,9, Leu11-SP; D-Pro4, D-Trp7,9,10-SP(4–11), respectively. lumbosacral section bound 3 more cervical thoracic sections, although 0.06 that sections. data suggest one class site indicate direct role functions.
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