Microbial oil-degradation under mild hydrostatic pressure (10 MPa): which pathways are impacted in piezosensitive hydrocarbonoclastic bacteria?

摘要: Oil spills represent an overwhelming carbon input to the marine environment that immediately impacts sea surface ecosystem. Microbial communities degrading oil fraction eventually sinks seafloor must also deal with hydrostatic pressure, which linearly increases depth. Piezosensitive hydrocarbonoclastic bacteria are ideal candidates elucidate impaired pathways following at low In present paper, we tested two strains of ubiquitous Alcanivorax genus, namely A. jadensis KS_339 and dieselolei KS_293, is known rapidly grow after spills. Strains were subjected atmospheric mild pressure (0.1, 5 10 MPa, corresponding a depth 0, 500 1000 m, respectively) providing n-dodecane as sole source. Pressures equal 10 MPa significantly lowered growth yields both strains. However, in strain KS_293 grown CO2 production per cell was not affected, integrity preserved PO43− uptake increased. Analysis its transcriptome revealed 95% genes downregulated. Increased transcription involved protein synthesis, energy generation respiration pathways. Interplay between these factors may play key role shaping structure microbial developed limit their bioremediation potential.