Dystrophin Immunohistochemistry in Muscular Dystrophies on Formalin Fixed Paraffin Embedded Tissue
作者： Saira Sajid , Abdul Hannan Nagi , Tanveer Ahmad , Maham Akhlaq , None
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摘要: Muscle biopsy with proper immunohistochemistry (IHC) is the gold-standard to distinguish muscular dystrophies (deadly degenerative disorders). Immunohistochemistry detects mutated proteins of dystrophies. Presently IHC performed on fresh frozen muscle specimens. This study was 32 children suffering from various types Among them 71.87% (n = 23) were diagnosed as DMD, whereas 31.25% 10) BMD and remaining one case (3.12%) LGMD. Nine patients females. It shows that can also be formalin fixed paraffin embedded tissues. In this comprising 23 males 9 females, we used blocks skeletal tissue using dystrophin β-spectrin antibodies in diagnosis Pakistan still dependent clinical features creatine phosphokinase (CPK) values. first subcontinent performing successfully formaline (FFPE) for
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