Role of interleukin-6 in the proliferation of human multiple myeloma cell lines OCI-My 1 to 7 established from patients with advanced stage of the disease
作者： MD Minden , HA Messner , JK Hitzler , H Martinez-Valdez , DB Bergsagel
DOI: 10.1182/BLOOD.V78.8.1996.1996
关键词:
摘要: Interleukin-6 (IL-6) has been shown to stimulate the proliferation of multiple myeloma cells purified a high degree from human bone marrow. IL-6 production in attributed belonging clone, thus supporting mechanism autostimulation. In addition, it that may be produced by auxiliary cell populations marrow are not part clone. A definitive separation both putative sources for difficult achieve fresh patient growth requirement and pure using seven lines (OCI-My 1 7) were established patients with advanced disease. The proliferative response each line recombinant was measured clonogenic assay providing plasma methylcellulose as viscous support 3H-thymidine uptake liquid suspension culture. We observed marked heterogeneity, ranging IL-6-dependent colony formation OCI-My 4, IL-6-independent growth. All expressed mRNA receptor. Expression analyzed after amplification polymerase chain reaction present five lines. protein detected enzyme-linked immunosorbent (ELISA) culture supernatants two 3 2). Its functional activity confirmed bioassay murine hybridoma B 13.29. This neutralized anti-IL-6 antibody. Two did express IL-6. remaining three IL-6, but secrete protein. Immunoprecipitation experiments lysates one these detect presence These results suggest autocrine stimulation occur some derived myeloma. However, does represent universal
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