Determination of Phosphates in Cardiac Troponin by Phosphate Analysis and Phosphoserine Modification

作者: Kristine Swiderek , Kornelia Jaquet , Helmut E. Meyer , Ludwig M. G. Heilmeyer

DOI: 10.1007/978-1-4757-0166-1_16

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摘要: Troponin (Tn) of high endogenous phosphate content (ca. 3 mol phosphate/mol protein) was isolated from beaf heart. Following separation Tn into its subunits T, I and C by reverse phase HPLC a about 1.4 protein is found in the T subunits, respectively. These results show, that cardiac contains at least four phosphorylation sites.

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