Thermal Injury and Recovery of Bacillus subtilis

摘要: Exposure of Bacillus subtilis NCTC 8236 to sublethal temperatures produced a change in the sensitivity organism salt and polymyxin. After 30 min at 47 C, 90% population was unable grow on modified sulfite polymyxin sulfadiazine agar containing an added 1% NaCl, glucose, asparagine. The data presented demonstrate that thermal injury results degradation both 16S 23S ribonucleic acid (RNA) damage cell membrane, suggested by leakage into heating mestruum material absorbing 260 nm. When cells were placed recovery medium (Trypticase soy broth), complete recovery, indicated returned tolerance polymyxin, occurred within 2 hr. presence protein inhibitor (chloramphenicol) wall inhibitors (vancomycin penicillin) during had no effect, whereas RNA (actinomycin D) effectively inhibited recovery. Further demonstrated injured able resynthesize species ribosomal using fragments which resulted from process. Also, precursor particles accumulated maturation not affected chloramphenicol medium.