Peripheral blood Th17 / Treg ratio increases in late asthmatic response

摘要: Background The late asthmatic response follows the early to allergen inhalation challenge (AIC) in half of atopic asthmatics (dual responder, DR). DRs develop airway hyperresponsiveness (AHR) and more prominent sustained inflammation after AIC. While Th17 regulatory T (Treg) cells have been studied asthma, their roles not fully understood isolated responders (ERs) DRs. A new method has utilized quantify immune cell subsets based on DNA methylation. Aims We aimed measure gene expression profiles blood pre post AIC, comparing ERs Methods Eight 6 underwent Blood were collected pre- 2 hours post-challenge. was used for epigenetic counting (Epiontis, Germany). Treg counted as percentage demethylation cell-specific region; IL17A FOXP3 Treg-specific demethylated regions, respectively, Th17/Treg ratio compared between using t-test. Gene measured with Affymetrix Human 1.0 ST array (Affymetrix, USA). After normalization, identified genes significantly correlated each cell-type. GeneGo network analysis performed biological functions. Results counts enriched analyses functions cellular functions, respectively. higher at baseline (p 0.002). increased 0.023). Conclusion imbalance may contribute development phase bronchoconstriction associated AHR inflammation.