Anti-cancer activity of DHA on gastric cancer—an in vitro and in vivo study

摘要: Treatment of gastric cancer remains a major challenge, and new anticancer drugs are urgently required. This study investigated whether dihydroartemisinin (DHA), semi-synthetic derivative artemisinin, could inhibit the growth both in vitro vivo. A series experiments including MTT, colony-forming, wound healing, invasion, cell cycle, cellular senescence, apoptosis assays were performed to examine antiproliferative antimetastatic effects DHA on three lines, SGC-7901, BGC823, MGC803. The result showed that proliferation rate colony-forming abilities cells significantly suppressed by together with significant suppression expressions markers (PCNA, cyclin E, D1), upregulation p21 p27. Moreover, induced G1 phase cycle arrest hindered migration invasion corresponding downregulation MMP-9 MMP-2. Furthermore, through suppressing Bcl-2 as well activating caspase-9 PARP. increased miR-15b miR-16 expression, caused Bcl-2, resulting cells. In vivo, our data inhibited SGC7901 cell-transplanted tumors. summary, we have shown is able metastasis human cancer. modulation mediated Our work suggested has against vivo vitro, indicating it promising therapy for