Assay of Deoxyhypusine Hydroxylase Activity
作者： Jong Hwan Park , Edith C. Wolff , Myung Hee Park
DOI: 10.1007/978-1-61779-034-8_13
关键词:
摘要: The eukaryotic translation initiation factor 5A (eIF5A) is the only cellular protein that contains an unusual amino acid, hypusine [Ne-(4-amino-2-hydroxybutyl)-lysine]. eIF5A and its hypusine/deoxyhypusine modification are vital for cell proliferation. Hypusine formed posttranslationally by two enzymatic steps catalyzed deoxyhypusine synthase hydroxylase. Deoxyhypusine hydroxylase catalyzes a stereo-specific hydroxylation of residue in intermediate protein, eIF5A(Dhp). enzyme totally specific this does not act on short peptides (<50 acids). assay measures conversion radiolabeled to eIF5A. Optimum conditions reaction detection methods product, hypusine-containing eIF5A, described chapter. first, most reliable, method measurement amount [3H]hypusine hydrolysate after separation from [3H]deoxyhypusine, ion exchange chromatography. This require specialized equipment. second based counting total TCA soluble radioactivity sodium periodate oxidation mixture, since 4-amino-2-hydroxy butyl moiety cleaved released as β-propionaldehyde formaldehyde oxidation.
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