The influence of clinical severity and topical antimicrobial treatment on bacteriological culture and the microbiota of equine pastern dermatitis.

摘要: BACKGROUND Equine pastern dermatitis (EPD) is a common dermatological problem in horses, yet its aetiology and pathogenesis are poorly understood. OBJECTIVES This study aimed to investigate the effects of lesion severity topical antimicrobial treatment on bacterial flora EPD-affected skin. ANIMALS Sixteen horses with EPD were investigated. METHODS AND MATERIALS An observational was conducted by assigning clinical score ranging from 0 (macroscopically nonlesional) 21 (severe), sampling most least severely affected limbs 16 (32 limbs) for bacteriological culture 16S rRNA sequencing. Topical month before recorded. The allocated nonlesional or mildly group (Group A, 0-3) moderate B, 4-21). RESULTS The commonly cultured species Staphylococcus aureus (one 15 Group A versus nine 17 B). Within S. aureus found three six treated antimicrobials 11 untreated limbs. β-haemolytic streptococci (three 32) Trueperella pyogenes (two also exclusively B. Staphylococci more often sequencing than culture. Limbs higher appeared have lower alpha diversity different beta compared milder lesions. CONCLUSIONS CLINICAL IMPORTANCE Observed differences microbiota equine skin likely be linked presence treatment. Further research needed establish causal bacteria.