A Comprehensive Study of the Effects of Neurotoxins on Noradrenergic Phenotypes, Neuronal Responses and Potential Intervention by Antidepressants in Noradrenergic Cells
作者： Yan Wang
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摘要: DBH and NET are the noradrenergic phenotypes for their functional importance to neurons. It is known that in vivo DSP4 treatment induces degeneration of terminals by interacting with depleting intracellular norepinephrine. However, DSP4’s precise mechanism action remains unclear. In this study various biochemical approaches were employed test hypothesis down-regulates expression NET, determine molecular mechanisms may be involved. The results showed SH-SY5Y neuroblastoma cells significantly decreased mRNA protein levels NET. DSP4-induced reduction levels, as well a timeand concentration-dependent manner. Flow cytometric analysis demonstrated DSP4-treated arrested predominantly S-phase, which was reversible. arrest confirmed several DNA damage response markers (phosphorylation H2AX p53),
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