Insulin receptor knockdown blocks filarial parasite development and alters egg production in the southern house mosquito, Culex quinquefasciatus.

摘要: Lymphatic filariasis, commonly known as elephantiasis, is a painful and profoundly disfiguring disease. Wuchreria bancrofti (Wb) responsible for >90% of infections the remainder are caused by Brugia spp. Mosquitoes genera Culex (in urban semi-urban areas), Anopheles rural areas Africa elsewhere), Aedes Pacific islands) major vectors W. bancrofti. A preventive chemotherapy called mass drug administration (MDA), including albendazole with ivermectin or diethylcarbamazine citrate (DEC) used in endemic areas. Vector control strategies such residual insecticide spraying long-lasting insecticidal nets supplemental to core strategy MDA enhance elimination efforts. However, increasing resistance mosquitoes parasite limit effectiveness existing interventions, new measures needed mosquito population disruption mosquito-parasite interactions reduce transmission. Mosquito insulin signaling regulates nutrient metabolism has been implicated reduced prevalence intensity malaria parasite, Plasmodium falciparum, infection mosquitoes. Currently no data available assess how affects development multi-cellular parasites, filarial nematodes. Here, we show that receptor knockdown blood fed C. quinquefasciatus, vector Wb India, completely blocks nematode infective L3 stage, results decreased ecdysteroid production trypsin activity leading fewer eggs. These indicate functional (IR) necessary reproduction. Therefore, may represent target blocking strategies.