Acute, but not chronic, stress increased the plasma concentration and hypothalamic mRNA expression of NUCB2/nesfatin-1 in rats

摘要: Nesfatin-1, a newly discovered satiety peptide, has recently been reported to be involved in the stress response. Stress-induced expression of nesfatin-1 and few studies focus on its hypothalamus, which is center To test our hypothesis that peripheral hypothalamic overexpression should play an important role response associated hyperactivity hypothalamic-pituitary-adrenal (HPA) axis, acute (AS) was induced using water avoidance (WAS), chronic unpredictable mild (CUMS) also 3 consecutive weeks 7 different stressors. The behavior CUMS rats evaluated by open field (OFT), sucrose preference (SPT), forced swimming (FST). activity HPA axis detected measurement plasma corticosterone concentration mRNA corticotropin-releasing-hormone (CRH). were measured with enzyme-linked immunosorbent assay (ELISA) real-time fluorescent quantitative PCR, respectively. results showed both AS increased CRH expression. Depression-like rats, as indicated decreased movement distance, frequency rearing grooming OFT, index immobility FST. Moreover, nesfatin-1, positively correlated expression, These stress, but not NUCB2/nesfatin-1 rats.