Upstream gene of the mgl operon controls the level of MglA protein in Myxococcus xanthus.

摘要: The mgl operon contains two open reading frames (ORFs) which are transcribed together. A collection of nonmotile mutants helped to define the downstream ORF as mglA gene. Single mutations at locus completely abolish motility. series deletion was constructed determine role upstream (now called mglB). strain carrying a in mglB and with an intact produces small colonies. cells motile, but their rate swarm spreading is reduced. Measurements cell movement showed that mutant advanced, on average, less than 0.1 length 5 min. mglB+ advanced average 1.3 lengths same time. Extracts delta contain 15 20% much 22-kDa MglA protein do cells, measured Western immunoblots enzyme-linked immunosorbent assays. However, amount transcript strain. Heterozygous partial diploids mglB/mglA wild-type alleles trans have normal motility, demonstrating largest deletions not polar mglA. Like other motility defects, mutation alters fruiting body development sporulation. delayed aggregation, produced immature bodies, sporulated 45 50% levels. All aspects phenotype explained by reduced levels assumption it limits gliding.