Molecular Evidence for the Thriving of Campylobacter jejuni ST-4526 in Japan

摘要: Campylobacter jejuni is a leading cause of human gastroenteritis worldwide. This study aimed at better understanding the genetic diversity this pathogen disseminated in Japan. We performed multilocus sequence typing (MLST) isolated from different sources (100 human, 61 poultry, and 51 cattle isolates) Japan between 2005 2006. approach identified 62 types (STs) 19 clonal complexes (CCs), including 11 novel STs. These STs were phylogenetically divided into 6 clusters, partially exhibiting host association. ST (ST-4526) that has never been reported other countries; phylogenetic analysis showed ST-4526 related distant lineage founder ST, ST-21 within CC-21. Comparative genome was to investigate which properties could be responsible for successful dissemination Results revealed three representative isolates contained putative island comprising region Cj0737 Cj0744, differed reference strain NCTC11168 (ST-43/CC-21). Amino acid alignment analyses two expressed 693aa- filamentous hemagglutination domain protein (FHA), while most C. strains whose sequenced exhibited its truncation. Correspondingly, cell binding FHA-positive greater than FHA-truncated strains, exogenous administration rFHA reduced adhesion bacteria. Biochemical assays dose-dependent affinity heparan sulfate, indicating adhesin activity. Moreover, increased antibiotic-resistance (nalidixic fluoroquinolones) ability DNA uptake. Taken together, our data suggested these combined features contributed thriving